2008年5月14日 · When investigating differential gene expression, quantitative real-time PCR (qPCR) data of two or more different conditions (such as control/treatment or healthy/pathological) are compared by using the fluorescence data acquired by the hardware.

qpcR is an R package that can analyze sigmoidal qPCR (quantitative polymerase chain reaction) data by fitting various sigmoidal, mechanistic, exponential and spline models to the raw data.

2024年12月6日 · qPCR，全称Quantitative Polymerase Chain Reaction，即 定量聚合酶链式反应，是一种常见且较为重要的实验室技术，可以对目标DNA或RNA进行定性以及定量分析。 与传统的PCR不同，qPCR不仅能扩增DNA，还能实时监测扩增过程中的荧光信号变化，进而得出目标序列在样本中的数量。 qPCR的基本原理是在 PCR反应 中，通过添加 荧光染料 或 荧光探针，当DNA模板被扩增时，荧光信号会随之增加。 实时监测荧光信号的变化可以计算出 扩增曲线， …

2019年5月2日 · Model fitting, optimal model selection and calculation of various features that are essential in the analysis of quantitative real-time polymerase chain reaction (qPCR).

Here we develop a mathematical model, termed the Common Base Method, for analysis of qPCR data based on threshold cycle values (C q) and efficiencies of reactions (E).

Model fitting, optimal model selection and calculation of various features that are essential in the analysis of quantitative real-time polymerase chain reaction (qPCR).

Model fitting, optimal model selection and calculation of various features that are essential in the analysis of quantitative real-time polymerase chain reaction (qPCR). Please use the canonical form https://CRAN.R-project.org/package=qpcR to link to this page.

2012年8月16日 · The work I present here extends the kinetic approach to modeling qPCR by including primer concentration in the model and providing analytical equilibrium solutions for the re-association phase. I also introduce the use of simultaneous analysis of qPCR curves which have common values for rate constants in the kinetic models. Current models

Here, we introduce the R package qPCRtools, which enables users to analyze the efficiency of gene amplification. Additionally, this software can determine gene expression levels using one of three approaches: the conventional curve-based method, the 2 −ΔΔCt method, and the SATQPCR method.

Quantitative PCR (qPCR) is one of the foremost methods for detection and quantification of nucleic acid targets. The technique is also known as real-time qPCR and is not to be confused with RT-qPCR (Reverse Transcription-qPCR).