Simple and fast quantification of DNA damage by real-time PCR, …
2017年7月11日 · We describe a real-time (rt) PCR-based method of quantifying DNA damage, adapted from the long-run rtPCR method of DNA damage quantification (LORD-Q) developed …
Quantitative sensing and signalling of single-stranded DNA
2019年2月26日 · DNA damage elicits a signalling response termed the DNA damage checkpoint. Once activated, the checkpoint induces several global (cell-wide) changes to cell physiology, …
Development of a qPCR Method to Measure Mitochondrial and Genomic DNA ...
2016年10月8日 · Here we report on an adaptation of a qPCR technique to assess DNA damage in nuclear and mitochondrial targets relative to control. Novel aspects of this assay include …
Newly revised protocol for quantitative PCR-based assay to …
Given the crucial role of DNA damage in human health and disease, it is important to be able to accurately measure both mitochondrial (mtDNA) and nuclear (nDNA) damage. In this unit, we …
Analysis of gene-specific DNA damage and repair using
QPCR has been used to survey DNA damage induced by different genotoxicants and to establish the repair kinetics of numerous genes. Current work seeks to analyze damage and repair in …
Quantitative PCR-based measurement of nuclear and mitochondrial DNA …
In this chapter, we describe a gene-specific quantitative PCR (QPCR)-based assay for the measurement of DNA damage, using amplification of long DNA targets. This assay has been …
Quantification of DNA Damage by Real-Time qPCR - PubMed
This chapter describes the use of real-time qPCR to quantify damages in genomic DNA. The method is based on the ability of a lesion in one strand to inhibit restriction enzyme digestion …
Quantification of DNA Damage by Real-Time qPCR
Subsequent amplification of the complementary, damage-free DNA strand in a real-time quantitative qPCR allows quantitative assessment of DNA damage with high site specificity. …
Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA ...
QPCR can be used to quantify the formation of DNA damage, as well as the kinetics of damage removal. One of the main strengths of the assay is that it permits monitoring the integrity of …
ate when compared to the undamaged DNA (4). QPCR is a suitable method for the measuring damage and repair in the subgene level functional units l. ke promotor regions, exons and …