A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. [1][2][3] F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division.

细菌人工染色体（Bacterial artificial chromosome，BAC）是指一种以F质粒（F-plasmid）为基础建构而成的细菌染色体克隆载体，常用来克隆150kb左右大小的DNA片段，最多可保存300kb个碱基对。

5 天之前 · A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.

細菌人工染色體（Bacterial artificial chromosome，BAC）是指一種以F質體（F-plasmid）為基礎建構而成的載體，最多可保存300000個鹼基對（平均約150000）。

2024年7月24日 · Bacterial Artificial Chromosomes (BACs) offer a powerful tool in genetic research and biotechnology. Their primary importance stems from their ability to maintain and replicate large DNA fragments within bacterial cells, which is …

The bacterial artificial chromosome (BAC) system is useful for creating a representation of the genomes of target species. The system is advantageous in that it can accommodate exogenous inserts that are very large (>100 kilobases, kb), thereby allowing entire eukaryotic genes (including flanking regulatory regions) to be encompassed in a ...

2023年8月4日 · 以大肠杆菌致育因子为基础进行构建的，能在生物细胞中独立、稳定存在和遗传的人工重组DNA分子。 从20世纪90年代起开始发展。 1989年， 奥康纳[注] 等首次用染色体建 …

Bacterial artificial chromosomes (BACs) are large genomic clones that are stably maintained in Escherichia coli. Because they are frequently large enough to contain an entire gene and its associated regulatory elements, BACs can serve as a source of …

2001年3月9日 · 细菌人工染色体是一种新发展起来的DNA载体系统，它具有容量大、遗传特性稳定、易于操作等优点.在基因文库构建和基因功能分析等方面有广泛的应用.综述了近年来发展起来的对BAC进行分析和修饰的一些方法. As a new developmental vector system, the bacterial artificial chromosome (BAC) have several advantages: larger capacity, hereditary stability and …

Authoritative and cutting-edge, Bacterial Artificial Chromosomes, Second Edition seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies.