STORM is the most widely used SRM method, and it relies on the sequential activation and time-resolved localization of photoswitchable fluorophores to generate high-resolution images. To achieve a high-quality multicolor image, specific labeling is required using direct labeling, protein conjugates, or antibody staining.

Feb 8, 2023 · STORM, part of a family of single-molecule localization microscopy (SMLM) techniques, activate or excite only a small percentage of fluorophores at one time, reducing spatial overlap and allowing images with as little as 5 nm resolution. STORM combines photoswitchable dyes and a lower power activation laser, blinking or switching from dark or ...

The Rothenberg research team studies the mechanisms of enzymes and proteins that participate in repair of DNA damage leading to cancer, and develops new imaging methods that will enable them to visualize the behavior of individual biological molecules. STORM Microscopy is used to localize and track DNA as it replicates in the cell.

Jan 29, 2018 · We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used...

STochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution fluorescence image by combining precise localization information for individual fluorophores in complex fluorescent specimens.

Jul 5, 2017 · STORM routinely achieves a spatial resolution of 20 to 30 nm, a ten-fold improvement compared to conventional optical microscopy. Among all SR techniques, STORM offers a high spatial resolution with simple optical instrumentation and standard organic fluorescent dyes, but it is also prone to image artifacts and degraded image resolution due to ...

Unlike techniques that rely on post-acquisition computational reconstruction (such as STORM), STED provides real-time super-resolution images. This direct imaging capability simplifies the process and reduces potential artifacts introduced during image reconstruction.

Stochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution fluorescence image by combining precise localization information for individual fluorophores in complex fluorescent specimens.

May 5, 2017 · Statistical results demonstrate that super-resolution reconstructions is significantly improved by interpolating low-resolution STORM raw images and using point-spread-function-based measurement matrices with better RIPs. The analysis of publically accessible experimental data confirms this conclusion. 1. Introduction.

Jan 1, 2012 · This chapter provides a comprehensive overview of how reliable structural information can be obtained from super-resolution imaging based on stochastic photoswitching of organic fluorophores in fixed and living cells.