Here we provide an overview of protein purification strategies, including guidelines on choosing a purification method and example protocols for protein purification using affinity tags.

Here we provide an overview of protein purification strategies, including guidelines on choosing a purification method and example protocols for protein purification using affinity tags.

purification of the protein of interest. There are four basic steps of protein purification: 1) cell lysis, 2) protein binding to a matrix, 3) washing and 4) elution. Cell lysis can be accomplished a …

Intro to Protein Tags for Purification. Affinity tags are a selective and versatile way to purify your protein of interest. Use this introductory guide to learn about what tagging systems are …

Whether you need an affinity tag for protein purification, want to create a single fusion protein to be used in a variety of protein analysis methods, or add an endogenous tag using CRISPR …

HaloTag® Protein Purification Systems provide a rapid, reliable method for purification of proteins expressed in E. coli and mammalian cells. For E. coli, the HaloTag® protein tag enhances …

The HaloTag® Protein Purification System(a–d) is based on a unique tag, the HaloTag® protein, which allows covalent, efficient and specific capture of the proteins expressed in E. coli as N …

The primary consideration for plasmid purification is separation of plasmid DNA from the chromosomal DNA and cellular RNA of the host bacteria. A number of methods have been …

HisLink™ Resin can be adapted to many protein purification techniques including column chromatography, batch chromatography and automated methods such as FPLC. Figure 5 …

A simple, rapid protocol for the use of paramagnetic precharged nickel particles to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate of bacterial, insect or …