Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. [1]

2025年1月17日 · Luciferase, enzyme manufactured in the cells of certain organisms to control bioluminescence. The widespread bioluminescence of such living organisms as fireflies and bacteria is based on the oxidation of a luciferin molecule in …

2024年7月16日 · Luciferase is a group of enzymes that oxidize a substrate known as luciferin to produce light. 1 For bioluminescence, the firefly luciferase enzyme catalyzes the oxidation of D-luciferin in the presence of oxygen (O 2), adenosine triphosphate (ATP), and …

Luciferase is an enzyme found in fireflies that facilitates the conversion of luciferin to oxyluciferin in the presence of ATP, O2, and Mg2+, resulting in the emission of yellow light. It is commonly used to detect low levels of gene expression due to its …

2024年4月16日 · Luciferase enzymes regulate bioluminescence in cells and can be found in a variety of organisms. It provides a useful research tool that allows for the study of gene regulation, among other things, and offers an alternative method to fluorescent techniques. Check out this DECODED article to find out what it is and how it can be used in the lab.

Luciferases are a heterogeneous group of oxidative enzymes that catalyze a reaction that produces an excited state intermediate that decays with the production of a photon.

2012年1月13日 · Luciferase is an enzyme that produces bioluminescence via the oxidation of a substrate, often referred to as luciferin. Many species of bacteria and eukaryotes make luciferases natively.

Learn more about luciferases, a class of enzymes used in bioluminescence, and how they are commonly used in reporter plasmids.

2018年5月23日 · Luciferase — An enzyme that catalyzes the reaction between oxygen and luciferin. Luciferin — Complex carbon molecules that produce light when oxidized. Oxidation — The process where a molecule loses one or more electrons.

Understanding luciferase enzymology and the structure of compounds that modulate luciferase activity can be used to improve the design of luminescence-based assays. This review provides an overview of these popular reporters with an emphasis on the ...