2021年9月24日 · Using Xenopus egg extracts, we define a three-step G4 unwinding mechanism that acts during DNA replication. First, the replicative helicase composed of Cdc45, MCM2-7 and GINS (CMG) stalls at a leading strand G4 structure.

The study of the genomic locations and biological consequences of G-quadruplex DNA is revealing fascinating insights into the regulation of DNA replication that are likely generalizable to other secondary structures and causes of replication stress.

2019年9月22日 · This mini-review will summarize recent advances in understanding the many strategies nature has evolved to overcome G-quadruplex-mediated replication blockage, including removal of the structure by helicases or nucleases, or circumventing the deleterious effects on the genome through homologous recombination, alternative end-joining or ...

G4 structures can induce DNA damage and block efficient DNA repair, but they can also support the activity and function of certain repair pathways. In this review, we highlight the roles and consequences of G4 DNA structures for DNA repair initiation, processing, and the efficiency of various DNA repair pathways.

2019年1月29日 · G4s can form from four strands of DNA (a tetramolecular G4, a form frequently studied in vitro but with questionable in vivo significance), two strands (a bimolecular G4, which can link two DNA strands together) and, most relevant in the context of DNA replication, a unimolecular G4 formed by folding of a single strand of DNA (Figure 1).

2019年9月22日 · The study of the genomic locations and biological consequences of G-quadruplex DNA is revealing fascinating insights into the regulation of DNA replication that are likely generalizable to other secondary structures and causes of replication stress.

2020年4月20日 · R-loops and G4s can form on opposite DNA strands (Figs 3b,4c), and then threaten genome stability by blocking DNA and RNA polymerases and causing transcription–replication conflicts.

2020年10月8日 · In DNA replication, replication timing during S phase is regulated by an evolutionally conserved Rif1 protein which specifically binds to G4. Rif1 may regulate chromatin architecture by tethering chromatin fiber through binding to multiple G4.

2016年11月1日 · G-quadruplexes (G4s), DNA secondary structures displaying noncanonical Watson–Crick base pairing, have recently emerged as key controllers of genome duplication. Here we discuss the various means by which G4s affect this fundamental cellular process.

2024年6月24日 · Here we discuss the interconnected functions of DNA:RNA hybrids and G4s within R-loops, their impact on DNA repair and gene regulatory networks, and their emerging roles in genome organization...