DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three [1] mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening.The cells are competent and often used with calcium chloride transformation to insert the desired plasmid.

Discover DH5α competent cells—the workhorse strain for general cloning and specialty cloning applications.

coli strains used to maintain and amplify small plasmid DNA is K-12 derived DH5α. Typical DNA assembly and cloning procedures involve as their last step transformation of the constructed plasmid into competent DH5α cells.

Thermo Scientific™ DH5α Competent Cells are ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.

NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. High efficiency strain ideal for a wide variety of applications

2012年12月20日 · See Help:Strains for more iformation about strains in EcoliWiki.. Alternate Names Sources Chromosomal Genotype. fhuA2 lac(del)U169 phoA glnV44 Φ80' lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17. Plasmids

Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors.

Escherichia coli K-12 DH5α is one of the most popular and widely available laboratory strains, but, surprisingly, no complete genome sequence has been publicly available. Here, we report the complete, finished sequence of NEB 5-alpha (DH5α fhuA2 ).

These two plasmids were retransformed into E. coli DH5α for confirmation of their beneficial effects on growth of E. coli in minimal media. The newly transformed strains showed growth phenotypes almost identical to those of the previously isolated transformants.

E. coli DH5α Competent Cells, prepared by Hanahan's method modified by Takara Bio, is a host for transformation. It can be used for Blue / White screening utilizing the activity of β-galactosidase (α-complementation) in combination use of pUC vec-tors. As this strain does not carry lac lq, basically IPTG is not needed. Therefore, E. coli