Genomic library - Wikipedia
A genomic library is a collection of overlapping DNA fragments that together make up the total genomic DNA of a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA.
Human Chromosome–Specific DNA Libraries: Construction and
1986年6月1日 · The goal of the National Laboratory Gene Library Project at the Los Alamos and Lawrence Livermore National Laboratories is the production of chromosome–specific human gene libraries and their...
Selection of chromosomal DNA libraries using a multiplex
2014年8月19日 · We developed quantitative gene assembly and DNA library insertion into the Saccharomyces cerevisiae genome by optimizing an efficient single-step and marker-free genome editing system using CRISPR-Cas9.
We describe the construction of two complete sets of small insert, complete di- gest DNA libraries for each of the 24 hu- man chromosomal types by the National Laboratory Gene Library Project. Flow sorting was used to purify the chromo- somes which provided the DNA for clon- ing.
Pure chromosome-specific PCR libraries from single sorted ... - PNAS
1994年6月21日 · Chromosome-specific DNA libraries can be very useful in molecular and cytogenetic genome mapping studies. We have developed a rapid and simple method for the generation of chromosome-specific DNA sequences that relies on polymerase chain reaction (PCR) amplification of a single flow-sorted chromosome or chromosome fragment.
Selection of chromosomal DNA libraries using a multiplex CRISPR …
2014年8月19日 · We developed quantitative gene assembly and DNA library insertion into the Saccharomyces cerevisiae genome by optimizing an efficient single-step and marker-free genome editing system using CRISPR-Cas9.
Human chromosome-specific DNA libraries: use of an …
1987年1月1日 · Libraries are being prepared for each human chromosome as part of the National Laboratory Gene Library Project and the specifications of seven new libraries are presented.
Genomic DNA Libraries - SpringerLink
Genomic DNA libraries are a collection of DNA fragments that together represent the entire (or nearly entire) genome of the mdividual from which the DNA was derived. These fragments are contained within self-rephcating vectors that enable them to be mamtamed and...
Construction and characterization of genomic libraries from …
The construction of chromosome-specific libraries by this method has the advantage of being direct and applicable to nearly all human chromosomes and will be important in molecular analysis of human genetic diseases.
Construction and Characterization of Chromosomal DNA Libraries …
Recombinant DNA libraries representing chromosomes 22 and 21 have been constructed with a view to studying the specific rearrangements of chromosome 22 observed in chronic myeloid leukaemia and Burkitt’s lymphoma and also the association of …