Nov 13, 2023 · We analyzed the accessible chromatin by assay using transposase-accessible chromatin with sequencing (ATAC-seq) in CFL1 -, YTHDC2 - or MLL1 -KO or non-KO PANC-1 cells to investigate the...

Aug 11, 2023 · We discovered the correlation between alterations in chromatin accessibility and the binding capabilities of m6A writers, erasers, and readers. The changes in chromatin accessibility influence the recruitment and activity of m6A regulatory proteins, thereby impacting the levels of m6A modification on specific mRNA transcripts.

Sep 7, 2022 · We show that RNA m 6 A guides DNA 5mC demethylation and thereby reprograms chromatin accessibility and gene transcription. Our results uncover a layer of epigenetic regulation in which RNA...

Mar 17, 2022 · Taken together, our findings suggest that MTC-mediated m 6 A modification protects nascent RNAs from Integrator-mediated termination and promotes productive transcription, thus unraveling an unexpected layer of gene regulation imposed by RNA m 6 …

Therefore, we have characterized a regulatory mechanism of chromatin accessibility and gene transcription mediated by RNA m 6 A formation coupled with DNA demethylation, highlighting the importance of the crosstalk between RNA m 6 A and DNA modification in physiologic and pathogenic process.

Nov 17, 2023 · We investigated seRNA m6A levels in pancreatic ductal adenocarcinoma (PDAC) and found that aberrantly increased m6A methylation promoted local chromatin accessibility, resulting in...

Mar 17, 2022 · This study investigated the chromatin-bound m 6 A regulators and provided evidence supporting the model where dynamic regulation of nascent RNA m 6 A methylation impacts nascent RNA synthesis.

Feb 2, 2023 · Here, we review RNA m6A modification in the light of the recent appreciation of nuclear roles for m6A in regulating chromatin states and gene expression, as well as the recent discoveries of the evolutionarily conserved methyltransferases, which catalyze methylation of adenosine on diverse sets of RNAs.

Mar 3, 2022 · The m6A MTC regulates nascent RNA synthesis in MCF-7 cells A-C. Aggregation plots (A), Snapshots of UCSC genome browser (B) and Boxplots (C) showing the nascent RNA level decreases upon METTL3 KD over Pre-mRNAs in MCF-7 cells. PRO-seq signal at TSS to TSS+100 region was calculated and Paired t-test was used for in C.

Jul 13, 2021 · Recently, several studies from different groups found that m 6 A occurred in chromatin associated regulatory RNAs (carRNAs), including promoter-associated RNA (paRNA), enhancer RNA (eRNA) and RNA transcribed from transposable elements (repeat RNA), which in turn influenced chromatin environment and transcription (Li et al., 2020; Liu et al ...