CRISPRi-based circuits to control gene expression in plants
2024年5月20日 · Here, we implement a CRISPR (clustered regularly interspaced short palindromic repeats) interference (CRISPRi)-based reversible gene circuit platform in plants. We create a toolkit of...
CRISPR screens in plants: approaches, guidelines, and future …
In this review, we discuss the principles and methods for building high-throughput CRISPR screening platforms as well as the specific challenges and advantages when implementing this technology in plants. We describe recent studies that have used this approach to build plant knockout mutant collections or to engineer and evolve new genetic ...
CRISPR/Cas9 in plant biotechnology: applications and challenges
In this review, recent progress in utilizing CRISPR/Cas9-mediated gene editing in plants to enhance certain traits in beneficial crops, including rice, soybean, and oilseed rape, is discussed. In addition, novel methods of applying the CRISPR/Cas9 system in live cell imaging are also extensively reviewed.
CRISPR screens in plants: approaches, guidelines, and future
2021年5月31日 · Here we discuss the general concepts, tools, and workflows for establishing CRISPR screens in plants and analyze the handful of recent reports describing the use of this strategy to generate mutant knockout collections or to diversify DNA sequences.
CRISPR/Cas9-Based Genome Engineering in Plants for Enhancing …
3 天之前 · In plants, the CRISPR process includes target site selection, synthesis of sgRNA, and the subsequent screening of gene-edited plants, enabling various genome editing abilities like knock-in, knockout, and knockdown (Endo et al. 2016). The continuous exploration of CRISPR, along with efforts to broaden PAM sequences, promises further ...
An efficient CRISPR-Cas12a-mediated MicroRNA knockout strategy in plants
2024年10月14日 · In this study, we developed an effective CRISPR-Cas12amediated miRNA knockout system to accurately generate loss-of-function miRNA mutants in plants. As a proof of concept, we demonstrated the Cas12a nuclease in rice to efficiently generate loss-of-function mutants in 10 different miRNA genes ( OsMIR390, …
Targeted gene knockout via CRISPR/Cas9: precise genome
2023年11月9日 · Our study demonstrates the successful use of the CRISPR/Cas9 system to generate PDS knockout mutants in eggplant, which has potential applications in plant biotechnology. This work will be beneficial for functional genomics research in eggplant, and the CRISPR/Cas9 system can be used for generating desirable traits in eggplant.
Multi-Knock—a multi-targeted genome-scale CRISPR toolbox to …
2023年3月27日 · In this Article, we describe the development and validation of Multi-Knock, a novel genetic approach in plants that combines forward genetics with dynamically targeted genome-scale CRISPR/Cas9...
Updates and Applications of CRISPR/Cas Technology in Plants
This paper summarizes the new variants of the CRISPR/Cas system and applications of the CRISPR system in plant science for the enhancement of various attributes such as yield, quality, nutritional factors, stress tolerance, and herbicide tolerance through …
Boosting plant genome editing with a versatile CRISPR-Combo …
2022年5月20日 · Here we develop a versatile CRISPR-Combo platform, based on a single Cas9 protein, for simultaneous genome editing (targeted mutagenesis or base editing) and gene activation in plants. We...