Electron microscopy proved that bacteriophages are particulate and viral in nature, are complex in size and shape, and have intracellular development cycles and assembly pathways. The principal contribution of electron microscopy to bacteriophage research is …

2021年1月31日 · Electron microscopy of three well-studied families in the order Caudovirales: Myoviridae, Podoviridae, and Siphoviridae. These images are of negatively stained specimens – the most common EM technique used in phage studies.

Phage types identified by early electron microscopy include enterobacteriophages T4, T1, T7, T5, 7–11, ViI and Pseudomonas phage PB1. This paper retraces the development of early virus electron microscopy till the introduction of negative staining. Keywords: bacteriophage, electron microscopy, history.

Transmission electron microscopy (TEM) uses a beam of electrons to produce 1000x higher resolution compared to traditional light microscopes. Increased resolution (down to 0.2 nm) is enough to visualize even viruses.

2012年1月1日 · Electron microscopy proved that bacteriophages are particulate and viral in nature, are complex in size and shape, and have intracellular development cycles and assembly pathways. The principal contribution of electron microscopy to bacteriophage research is the technique of negative staining.

2018年11月1日 · Direct imaging of viral particles only became possible after the advent of the transmission electron microscope, allowing the determination of viral morphological characteristics.

2024年7月1日 · There are various ways to detect bacteriophages, and one such method is through the use of a transmission electron microscope (TEM). TEM can help in identifying families of bacteriophage (Ács et al., 2020) and it can also classify new viruses in novel families (Ackermann, 2012).

Negative staining of purified viruses is the most important electron microscopical technique in virology. The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages.

The visualization of viral particles only became possible after the advent of the electron microscope. The first bacteriophage images were published in 1940 and were soon followed by many other publications that helped to elucidate the structure of the particles and their interaction with the bacterial hosts.

Negative staining of purified viruses is the most important electron microscopical technique in virology. The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts.