手把手教学——酵母双杂交(Y2H)实验操作指南 - 知乎专栏
酵母双杂交系统(Yeast two hybrid，Y2H) 常用于分析两种蛋白质的相互作用。 将两种蛋白质分别克隆到酵母表达质粒的转录激活因子的DNA结合结构域（DNA-BD）和转录激活域（AD） …
pGBKT7 Sequence and Map - SnapGene
Yeast two-hybrid "bait" vector for expressing proteins fused to the GAL4 DNA-binding domain. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: Yeast Plasmids | More …
A novel, easy and rapid method for constructing yeast two-hybrid ...
2018年4月19日 · Here, we describe modification of the MCS region in the yeast plasmid pGADT7 to match that of pGBDT7 by site-directed mutagenesis, enabling easy and fast construction of …
the “prey” proteins are cloned into specific recombination-amenable two-hybrid vectors. We describe the construction of a reading frame–independent vector system for Y2H PPI stud-ies. …
In this study, we modified the multiple cloning site sequence of the yeast plasmid pGADT7 by site-directed mutagenesis PCR to generate the pGADT7-In vector, which result in an easy and …
2008年12月5日 · The pGBKT7 vector expresses proteins fused to amino acids 1–147 of the GAL4 DNA binding domain (DNA-BD). In yeast, fusion proteins are expressed at high levels from the …
To make your GAL4 DNA-BD/bait construct, we recommend using pGBKT7, which is available separately (Cat. No. 630443) or as a component of our Matchmaker Gold Two-Hybrid System …
pGBKT7-Y: Generate this construct by cloning the ORF cod-ing for the protein of interest Y in frame with the GAL4 BD in the pGBKT7 vector. pGADT7-TAg and pGBKT7-P53 is a well …
The construction of yeast two-hybrid bait vector pGBKT7-RARα-V
Objective To construct the bait ex pression vector pGBKT 7-RARA-V of retinoic acid receptor variant protein for screening the target proteins interacting with the bait protein through the …
Yeast two-hybrid vectors - Takara Bio
pGBKT7 is a yeast two-hybrid bait expression vector that is designed to express a fusion protein of the GAL4 DNA-binding domain (DNA-BD; amino acids 1–147) and a bait protein. The fusion …