L-threose is a product of ascorbate oxidation and degradation. By virtue of its free aldehyde group it can form Schiff-bases with tissue proteins, altering their normal function. In this study, we have examined the possibility of its detoxification to L-threitol by aldose reductase in the lens.

1996年6月21日 · L-threose is a product of ascorbate oxidation and degradation. By virtue of its free aldehyde group it can form Schiff-bases with tissue proteins, altering their normal function. In this study, we have examined the possibility of its detoxification to …

1994年6月1日 · l-Threose is a significant degradation product of ascorbic acid at pH 7·0 in the presence of oxygen. When compared to several other ascorbate-derived degradation products, it had the greatest ability to glycate and crosslink lens proteins in vitro.

2005年1月1日 · L-Threose was an excellent substrate for purified aldose reductase measured both spectrophotometrically and by the formation of L-threitol by TLC. Human lens contains AR activity, even in the lens core. When L-threose was incubated with increasing lens extracts, both protein incorporation and threitol formation were increased.

1996年6月21日 · L-threose is a product of ascorbate oxidation and degradation. By virtue of its free aldehyde group it can form Schiff-bases with tissue proteins, altering their normal function. In this study, we have examined the possibility of its detoxification to …

Key Takeaway: Aldose reductase in the rat lens can detoxify L-threose, potentially preventing protein glycation and toxicity due to ascorbate degradation products.

2005年12月31日 · L-Threose was an excellent substrate for purified aldose reductase measured both spectrophotometrically and by the formation of L-threitol by TLC. Human lens contains AR activity, even in the...

L-threose is a product of ascorbate oxidation and degradation. By virtue of its free aldehyde group it can form Schiff-bases with tissue proteins, altering their normal function. In this study, we have examined the possibility of its detoxification to L-threitol by aldose reductase in the lens.

2013年9月3日 · Aldose reductase (AR) is an NADPH-dependent reductase, which acts on a variety of hydrophilic as well as hydrophobic aldehydes. It is currently defined as the first enzyme in the so-called polyol pathway, in which glucose is transformed into sorbitol by AR and then to fructose by an NAD + -dependent dehydrogenase.

1994年6月1日 · L-Threose degrades very slowly in 0.1 M phosphate buffer at pH 7.0, but the addition of a four-fold excess of N alpha-acetyl-L-lysine accelerated the rate of disappearance of threose 30-fold, indicating a rapid glycation reaction.