Single-nucleus RNA sequencing of plant tissues ... - Wiley Online Library
2021年8月13日 · Single-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant …
Sample and library QC - Single Cell Discoveries
At Single Cell Discoveries, our lab team uses the Agilent 4200 TapeStation System. TapeStation plots will provide information about RNA or DNA fragments on two things: Keep in mind that …
Examples of Library Profiles Obtained on the TapeStation …
We have used single-nucleus RNA sequencing to extract transcriptomic information from multiple Drosophila tissues. Here, we describe procedures for the (1) dissociation of single nuclei,...
Example of Final mRNA Library TapeStation Trace - ResearchGate
By including oligonucleotide-labeled antibodies into high-throughput single-cell RNA-sequencing protocols, combined transcript and protein expression data can be acquired on the...
Low-cost and High-throughput RNA-seq Library Preparation for …
Here, we present a tissue-to-library RNA-seq protocol, amenable to multiplexing with complete preparation of 96 samples in three days, with costs of less than £10 per sample.
Single-cell RNA-seq of maize meiocytes and pollen grains - Nature
2023年10月2日 · Here, we present a protocol to isolate single maize meiocytes and pollen grains for RNA-seq. We discuss how to identify and isolate each sample type under a microscope, …
Plant Single-Cell/Nucleus RNA-seq Workflow - Springer
2022年12月11日 · In this chapter, we discuss the best practices to manipulate plant biological samples before conducting single-cell/nucleus RNA-seq experiments, provide information …
Single-nuclei RNA-sequencing of plants - ResearchGate
2020年11月16日 · Single-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant …
PlantscRNAdb: A database for plant single-cell RNA analysis - Cell …
2021年6月7日 · We established a plant single-cell transcriptome database (termed PlantscRNAdb; http://ibi.zju.edu.cn/plantscrnadb/). PlantscRNAdb includes 26 326 marker …
Single-Cell Transcriptome Analysis in Plants: Advances and …
2021年1月4日 · In this review, we compare and evaluate recent pioneering studies using the Arabidopsis root model, which has established new paradigms for scRNA-seq studies in plants.