2022年1月1日 · Phloem protein 2 (PP2) is a protein having lectin properties that can be isolated from the phloem sap. Based on our previous proteomic study of phloem sap of Cucumis sativus, it was found that the expression of PP2 A1-like was significantly up-regulated under salt stress, which may be a molecular mechanism of plant adaptation to stress.This paper carried out the expression and purification of ...

2017年11月23日 · Data from many studies have shown multiple uses of MBP as a fusion partner to prevent inclusion body formation and to promote soluble expression and purification of proteins of interest 19,20,21 ...

2024年7月27日 · An unknown functional protein was screened through the Uniprot protein database, and its primary structure did not resemble existing proteins. It was an α-helical cationic polypeptide we named PFAP-1. The codon-optimized full-length PFAP-1 gene was synthesized and inserted into the prokaryotic expression vector pET-30a. The induced expression ...

2017年10月1日 · Results showed that our constructed pET30(+)/PDL1-ECD system efficiently produces desired recombinant protein with molecular weight of 38.1 kDa.The prokaryotic expression system provides an easy method to express PD-L1 extracellular domain that further facilitate the role of PD-1/PD-L1 binding inhibition and …

2.4. Prokaryotic Expression and Purification of PFAP-1 Protein. The verified recombinant expression plasmid was transferred into E. coli BL21 (DE3), induced by IPTG, and the bacteria were collected for SDS-PAGE analysis. The results showed that PFAP-1 could be significantly expressed under the induction conditions of 15 °C and 37 °C compared ...

2024年7月27日 · Molecular docking results revealed that the optimal docking fraction of PFAP-1 protein and ACE2 protein was −267.78 kcal/mol, with a confidence level of 0.913.

2024年7月27日 · Pinctada fucata meat is the main by-product of the pearl harvesting industry. It is rich in nutrition, containing a lot of protein and peptides, and holds significant value for both medicine and food. In this study, a new active protein was discovered and expressed heterogeneously through bioinformatics analysis. It was then identified using Western blot, molecular weight, and mass ...

1 天前 · The results showed that there were at least 200 transcription factors directly or indirectly interacting with ANLN protein, many of which were reported as oncogenic transcription factors, such as ...

3 天之前 · Right: uS5 forms an r-protein cluster in eukaryotes and archaea. The ... Recombinant protein expression and purification. E. ... All data are representative results from at least three independent ...

2016年5月28日 · Prokaryotic expression systems have been widely used to express proteins for structural studies. Such expression systems have the advantage of being economical, straightforward and fast. However, for many eukaryotic proteins and particularly protein complexes, bacterial expression systems do not pro …