This study demonstrates the high level of concordance between glass slides evaluated using light microscopy and digital whole-slide images for pathologic response assessments. Pathologists did not require measurement tools to generate robust %RVT values from slide annotations.

We aim to evaluate the relative quality of digital slides scanned at ×20 versus traditional glass slides by estimating intrapathologist discrepancy in detecting specific microscopic features and examining for variation in the objective magnification needed to …

2016年7月11日 · Light microscopy (LM) is considered the reference standard for diagnosis in pathology. Whole slide imaging (WSI) generates digital images of cellular and tissue samples and offers multiple advantages compared with LM. Currently, …

2019年10月10日 · Whole slide images acquired with a 20× scan typically have a resolution of 0.25 µm/pixel, which is comparable to examining a glass slide with light microscopy (LM). This kind of digital image allows the user to navigate the slide, zoom in and out, and annotate areas of the image that are of particular interest. 1

2020年2月14日 · It is useful to consider differences between glass slides viewed with a microscope and WSIs viewed on flat digital monitors. First, all pathologists working today trained and have practiced using light microscopy; this is the format they are most familiar with and likely the most comfortable.

2020年2月6日 · Digitizing glass slides has revolutionized the practice of anatomical pathology, improving the diagnostic accuracy, turn around time, and outreach service coverage. Using slide scanners, glass slides are converted into digital slides rapidly accessible to the pathologist.

In this study, three dermatopathologists examined 181 cases using traditional light microscopy and whole slide images and found that glass-WSI intraobserver concordance (86.9%, 95% confidence interval [CI] 83.7–89.6) did not differ from glass-glass intraobserver concordance (90.3%, 95% CI 86.7–93.1) in any statistically meaningful way.

The light-sheet microscopy approach achieves optical sectioning (rejection of out-of-focus light) by using a thin ‘selec- tive’ illumination plane, which generates fluorescence signal...

2020年10月1日 · To compare pathologists' primary diagnoses derived from WSI versus the standard microscope. Because WSIs differ in format and method of observation compared with the current standard glass slide microscopy, this study is critical to potential clinical adoption of digital pathology. Design.—:

In one particular study in which digital and glass slides from 600 cases were compared, the results showed a diagnostic accuracy of 94% with WSI versus 99% with light microscopy. 10 Several studies in recent years have demonstrated that primary histopathologic diagnoses can be rendered digitally using WSI. 11–18 Discrepancies in diagnoses ...