These transgenic mice express the mouse codon-optimized FLP recombinase under the direction of the mouse Pgk1, phosphoglycerate kinase 1, promoter. Transgene expression is detected in testis, ovary, brain, heart, liver and muscle by RT-PCR.

2020年3月3日 · In order to analyze the effects of increased microenvironmental TGFβ concentration in vivo, we developed a conditional transgenic mouse model (Flpo/Frt system) expressing bioactive TGFβ in...

Two of the most exciting and versatile genetic tools designed in the last 30 years are the Cre-lox and FLP-FRT technologies. Both allow the location and timing of gene expression to be closely regulated. This article briefly outlines how the two systems work and can be used in …

FlpO expression and activity largely recapitulates the endogenous Slc32a1 gene - it is restricted to GABAergic neurons. These mice may be used to generate conditional mutations for studying gain-or-loss of function and/or fate mapping related to GABAergic neuronal function.

2009年11月26日 · The new FLPo deleter mice largely facilitated our process of generating the GHR (growth hormone receptor) conditional knockout mice. The superior recombination efficacy of this FLPo deleter mouse line will be beneficial to the scientific community and particularly valuable for conditional gene manipulation in the pure C57BL/6 genetic background.

2021年5月8日 · Cre（Cyclization Recombination Enzyme）是一种重组酶，于 1981 年从 P1 噬菌体中被发现，其基因编码区序列全长 1029bp，为 38kDa 大小的、由 343 个氨基酸组成的多肽单体蛋白。 Cre 重组酶的 C-末端结构域包含催化活性位点，能够催化 DNA 分子中特定位点之间的重组。 LoxP 是 Locus of X-overP1 的缩写，是位于 P1 噬菌体中长度为 34bp 的一段序列，由两个 13bp 的反向回文序列和 8bp 的中间间隔序列共同组成，反向回文序列是 Cre 重组酶的识别和结 …

2024年4月19日 · The Na v 1.8 Ires−FLPo mouse is an excellent model as it spatially restricts FLPo expression to a very small number of tissues, such as the DRG, TG, nodose ganglia (NG), and the conduction tissue of the heart. Temporal control over the expression and activation of the effector molecules depends on the induction of Cre recombination.

Recently, a codon improved version of the Flpe site specific recombinase, termed Flpo, was reported as having greatly improved performance in mammalian cell applications. However, the degree of improvement could not be estimated because essentially no Flpe activity was observed. Here, we compare Flp …

2024年12月17日 · In this study, we generated a new Drd1-Flpo mouse that has functional Flp expression in D1DR+ neurons. The Drd1-Flpo mouse line can be readily combined with other transgenic Cre lines to target more precise neuronal populations, including subpopulations of D1DR+ neurons.

2024年4月28日 · We generated a Drd1-Flpo mouse line that expresses Flp recombinase in D1DR+ neurons. This novel Drd1-Flpo mouse facilitates investigation of specific roles of D1DR+ neurons in various brain areas including the striatum, frontal cortex, and cerebellum, and it provides an alternative to existing Drd1-Cre mice.