Chromatin: Structure and Microscopic Observation - Microbe Notes
2023年8月3日 · Chromatin is defined as a complex of RNA, DNA, and Protein observed in eukaryotic cells. Its prime function lies in the packaging of DNA molecules in a very long …
Chromatin expansion microscopy reveals nanoscale organization …
2023年7月6日 · In this work, we developed chromatin expansion microscopy (ChromExM) to visualize chromatin, transcription, and transcription factors in vivo. ChromExM of embryos …
ChromEMT: Visualizing 3D chromatin structure and compaction in …
2017年7月28日 · To visualize and reconstruct chromatin ultrastructure and 3D organization across multiple scales in the nucleus, we developed ChromEMT, which combines electron …
Chromatin conformation, gene transcription, and nucleosome
2025年1月10日 · The simplistic view that dense chromatin (heterochromatin) suppresses gene expression while loose chromatin (euchromatin) facilitates it fails to capture the complexity …
Super-Resolution Microscopy of Chromatin - PMC - National …
2019年6月28日 · With the aid of chromatin-specific fluorescence labeling techniques, we are gaining increasing insight into gene regulation and chromatin organization. Combined with …
Advanced microscopy methods for visualizing chromatin structure ...
2015年10月7日 · To visualize chromatin, one can either label chromatin-associated proteins such as the core histone proteins or the DNA itself. Chromatin associated proteins can be tagged …
ChromEMT: Visualizing 3D chromatin structure and compaction in ...
2017年7月28日 · Using ChromEMT (ChromEM tomography), we reveal the ultrastructure and three-dimensional (3D) organization of individual chromatin polymers, megabase domains, …
Analysis of three-dimensional chromatin packing domains by chromatin …
2022年7月16日 · Herein, we introduce ChromSTEM, a method that utilizes high-angle annular dark-field imaging and tomography in scanning transmission electron microscopy combined …
Ultrastructural visualization of 3D chromatin folding using volume ...
2020年5月1日 · Here, we report the development of an electron microscopy method that combines serial block-face scanning electron microscopy with in situ hybridization (3D-EMISH) …
Cryo-electron microscopy of the chromatin fiber - ScienceDirect
2020年10月1日 · Cryo- electro microscopy reveals the native conformation of chromatin fiber both in vitro and in situ. Presence of linker histone and ionic conditions determines the level of fiber …