Homogenization, in cell biology or molecular biology, is a process whereby different fractions of a biological sample become equal in composition.

2015年11月2日 · Homogenization is the name given to the methodological steps necessary for releasing organelles and other cellular constituents as a free suspension of intact individual components. Most homogenization procedures used for mammalian cells (e.g., cavitation pump and Dounce homogenizer) rely on mechani …

2024年3月6日 · The essence of cell homogenization lies in breaking down the cell walls, allowing researchers to delve deep into the cell's intricate workings. Precise homogenization …

Tissue homogenization is a critical process in biological research that allows scientists to break down complex tissues into homogeneous mixtures, enabling the isolation and analysis of specific components. It plays a crucial role in studying cellular functions, identifying disease markers, and developing therapeutic interventions.

Cell homogenization is the process of disrupting cell membranes to release their contents, creating a homogeneous mixture called a homogenate. This process is crucial for subsequent steps like cell fractionation, which involves separating the …

2015年11月1日 · Most homogenization procedures used for mammalian cells (e.g., cavitation pump and Dounce homogenizer) rely on mechanical force to break the plasma membrane and may be supplemented with osmotic...

The aim of the homogenization procedure must be to produce at least 90% cell breakage, reproducibly, under the mildest conditions. Clearly, homogenization under iso-osmotic conditions is an ideal which should be aimed for and an effective strategy for homogenizing monolayer cells under such conditions, based on the method of Marsh et al.[2], is ...

In this protocol, we describe a syringe-based homogenization method that does not require specialized equipment, is easy to handle, and gives reproducible results. The method may be adapted for cells that require hypotonic shock before homogenization.

2002年6月13日 · Satisfactory homogenization of cultured cells is a necessary prerequisite to any fractionation schedule. Protocols are given for homogenization in iso-osmotic (A) and hypo-osmotic (B) media that should be broadly applicable to any cell type and to any subsequent fractionation procedure.

This chapter covers the various methods of mechanical cell disruption and tissue homogenization that are currently commercially available for processing small samples s < 1 mL) to larger multikilogram production quantities.