A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. [1] [2] [3] F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division.

2 天之前 · A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.

Diagram of a typical BAC vector. This vector is a compilation of the original pBAC108L (Shizuya et al., 1992), pBACe3.6 (Frengen et al., 1999) and pCC1BAC™ (Epicentre Technologies). The cam® gene encodes chloramphenicol antibiotic resistance.

This protocol provides a general methodology applicable to cloning full-length cDNA for a range of positive-strand RNA viruses, particularly those with a genome of >10 kb in length, into a BAC vector, from which infectious RNAs can be transcribed in vitro …

2014年11月1日 · In-Fusion cloning was performed using the designer DNA fragment to modify pIB139, followed by λ-RED-mediated recombination to obtain the BAC-based expression vector. We demonstrated the effectiveness of this method by rapid construction of a BAC-based expression vector with an insert of about 120 kb that contains the entire gene cluster for ...

2008年3月15日 · pBeloBAC11 is a second-generation mini-F-based BAC cloning vector with lacZα for facilitating the detection of recombinants and the cat gene for transformant selection with chloramphenicol. The F factor carries genes that are essential for regulating its own replication and for copy number control in the cell.

2012年2月16日 · We use the term 'BIBAC' for large-insert DNA clones cloned in a transformation-competent, binary plasmid-based vector with a bacterial host, regardless of whether the target DNA is cloned in...

Development of BAC vectors The BAC vector was constructed 1 based on Escherichia coli Factor F’ (F’) since methods of DNA manipulation in bacteria are well established. F’ is an incompatibility group involved in E. coli chromosomal transfer and conjugative ability, which can exist as an extra-chromosomal element. The original BAC vector,

2024年7月24日 · Bacterial Artificial Chromosomes are sophisticated vectors designed to accommodate large DNA fragments. At their core, BACs are derived from a specific type of plasmid found in Escherichia coli, known as the F-plasmid. This plasmid is naturally equipped to handle large DNA sequences, making it an ideal backbone for BAC construction.

Fast accurate construct design for all major molecular cloning techniques; Validate sequenced constructs using powerful alignment tools; Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure