2020年9月27日 · The culture of stem cells in 3D may employ various matrices or scaffolds, in addition to the cells, to support the complex structures. The goal of this Special Issue is to bring together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cells and culture techniques, and also highlight stem cell ...

The culture of stem cells in 3D may employ various matrices or scaffolds, in addition to the cells, to support the complex structures. The goal of this Special Issue is to bring together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cells and culture techniques, and also highlight stem cell ...

2021年10月11日 · Three-Dimensional Stem Cell Culture Systems. 3D cell culture technology, which refers to the cocultivation of carriers with 3D structures of different materials and various types of cells in vitro, so that the cells can migrate and grow in the 3D structure of the carrier to form a 3D cell-carrier complex, can be divided into scaffold-free and ...

2024年10月1日 · Collaboration between experts in academia and industry has driven the development of stem cell-based products, including the introduction of automated manufacturing systems for large-scale production of clinical-grade tissues. 13 This review aims to elaborate on the establishment of scaffold-free 3D culture systems of stem cells and their ...

2017年11月1日 · Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics. Cell Stem Cell, 14 (2014), pp. 13-26. View PDF View article View in Scopus Google Scholar ... Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro. J. Anat., 227 (2015), pp. 746-756. Crossref View in ...

Eventually stem cells started to culture and overpopulate the biomaterial for degradation. ... (2015) Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D. Biofabrication 7: 044102.

2023年11月21日 · Here, Santos et al. compared the commitment potential of umbilical cord stem cells (a type of mesenchymal stem cell) into keratinocytes in a 3D in vitro skin model composed of dermal equivalent (collagen type I + dermal fibroblasts) or commercial porcine skin decellularised matrix, both in an air-liquid interface assay. The dermal equivalent ...

2021年4月14日 · In contrast, cells in 3D culture can make cell interactions in all directions, between the encompassing matrix and between neighboring cells 42. For this reason, 3D culture provides higher ...

2018年4月12日 · Stem cells remind us again and again of the importance of niche in cell culture. Pluripotent or omnipotent stem cells will neither expand, nor differentiate into desirable phenotypes, without the right environmental cues. Nature operates in three dimensions so the need for a three-dimensional (3D) environment for stem cell cultures should be ...

Modern 3D cell culture scaffold techniques often rely on the use of various biomaterials, including hydrogels. Hydrogels are hydrophilic, cross-linked polymer chains ... Stem cells are a fundamental tool for tissue engineering and regenerative medicine due to their pluripotency and differentiation ability. The potential of stem cells is limited ...