FZD5 controls intestinal crypt homeostasis and colonic Wnt …
2024年11月22日 · Mu et al. identify FZD5 as a critical regulator of intestinal stem cells and crypt homeostasis, underscoring its importance in potential therapeutic interventions for intestinal disorders.
Wnt specifically induces FZD5/8 endocytosis and degradation and …
3 天之前 · Figure 1. Wnt induces FZD5/8 endocytosis and degradation. (A) Wnt3a or Wnt5a specifically reduced the cell surface levels of V5-FZD5/8. HEK293A cells stably expressing each of the 10 V5-FZDs (FZD1 to 10) were treated with control, Wnt3a, or Wnt5a CM for 4 hours, and the cell surface levels of V5-FZDs were analyzed via flow cytometry using an anti-V5 antibody.
Next-Generation Surrogate Wnts Support Organoid Growth …
2020年11月5日 · Administration of Fzd subtype-specific NGS Wnt in vivo reveals that adult intestinal crypt proliferation can be promoted by agonism of Fzd5 and/or Fzd8 receptors, while a broad spectrum of Fzd receptors can induce liver zonation. Thus, NGS Wnts offer a unified organoid expansion protocol and a laboratory “tool kit” for dissecting the ...
FZD5 regulates cellular senescence in human mesenchymal stem/stromal cells
Although mesenchymal stem/stromal cells (MSCs) are promising for cell therapy, long‐term culture leads to MSC senescence with reduced stem cell capacity. We showed that FZD5, which is specifically expressed in immature MSCs, can serve as a stemness indicator. Controlling FZD5 signaling may improve MSC quality and the outcomes of cell therapy.
FZD5 controls intestinal crypt homeostasis and colonic Wnt …
2024年11月22日 · Here, an epithelial-specific Fzd5 knockout (KO) elicited lethal pan-intestinal crypt and villus loss, whereas an Lgr5+ ISC-specific Fzd5 KO depleted Lgr5+ ISCs via premature differentiation and repressed Wnt target genes. Fzd5-null phenotypes were rescued by constitutive β-catenin activation in vivo and in both mouse and human enteroids.
Frizzled receptors (FZDs) in Wnt signaling: potential therapeutic ...
2024年4月17日 · In HNSCC, FZD8 is highly expressed in cancer stem cells (CSCs) and is a crucial mediator of c-Met-maintained HN-CSCs, which are responsible for the recurrence and metastasis of HNSCC .
FZD5 controls intestinal crypt homeostasis and colonic Wnt
2024年11月24日 · KO of Fzd5, not Fzd8, in enteroids ablated responsiveness to dual-specificity FZD5/FZD8-selective Wnt surrogate agonists, which ameliorated DSS-induced colitis in wild-type and Fzd8 KO mice. Overall, FZD5 is a dominant and essential regulator of crypt homeostasis, Lgr5+ ISCs, and intestinal response to Wnt surrogate agonists, with implications ...
Single-cell transcriptomics identifies a WNT7A-FZD5 ... - Cell Press
We genetically label, isolate, and characterize putative FT stem cells using functional approaches as well as bulk and SCT analyses, identifying a hormonally regulated WNT7A-FZD5 signaling axis that is critical for FT stem cell maintenance.
FZD5 regulates cellular senescence in human mesenchymal stem/stromal cells
2020年12月20日 · We found that the transcription factor Frizzled 5 (FZD5) is expressed specifically in immature hMSCs. The FZD5 cell surface antigen was also highly expressed in the primary MSC fraction (LNGFR + THY-1 +) and cultured MSCs. Treatment of cells with the FZD5 ligand WNT5A promoted their proliferation.
WNT signaling in stem cell biology and regenerative medicine
FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, FZD9, FZD10, LRP5, LRP6, and ROR2 are transmembrane receptors transducing WNT signals based on ligand-dependent preferentiality for caveolin- or clathrin-mediated endocytosis.
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