Super-resolution imaging (SR) is a class of techniques that improve the resolution of an imaging system. In optical SR the diffraction limit of systems is transcended, while in geometrical SR the resolution of digital imaging sensors is enhanced.

2023年2月8日 · Super-resolution microscopy (SRM) encompasses multiple techniques that achieve higher resolution than traditional light microscopy. The resolution of conventional light microscopy is limited to around 200 nm due to the diffraction of light.

2018年8月31日 · Super-resolution imaging has enhanced our ability to extract dynamic information of cellular structures, allowing the mobility of biomolecules and the shape or structural dynamics of molecular complexes and organelles to be tracked with higher accuracy.

2019年1月2日 · Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable.

2024年12月1日 · We delve into the fascinating world of super-resolution imaging, specifically focusing on a technique known as Multi-Harmonic Structured Illumination-Based Optical Diffraction Tomography (MHSI-ODT).

2010年12月23日 · In this Primer, we review the technological advances in the burgeoning field of “super-resolution fluorescence microscopy.” Then, we describe the application of these techniques to various areas of biology, which have quickly demonstrated the great promise of this exciting new area of bioimaging.

Fluctuation-based techniques such as Super-resolution Optical Fluctuation Imaging (SOFI) and Super-Resolution Radial Fluctuations take advantage of natural variations in the intensity of light emitted by fluorophores as they transition through fluorescent and nonfluorescent states (Fig. 2D).

Image super-resolution (SR) is one of the vital image processing methods that improve the resolution of an image in the field of computer vision. In the last two decades, significant progress has been made in the field of super-resolution, especially by utilizing deep learning methods.

2024年10月4日 · Several fundamental approaches to achieving super-resolution SRS imaging have been reported, including optical engineering strategies, expansion microscopy, deconvolution image analysis, and photoswitchable SRS reporters as …

This system offers fast imaging with reduced pinhole crosstalk—ideal for live cell imaging and thicker specimens. The addition of super-resolution imaging allows researchers to surpass the diffraction limit, achieving resolution down to 120 nm.