Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded non-coding RNA molecules, typically 20–24 base pairs in length, similar to microRNA (miRNA), and operating within the RNA interference (RNAi) pathway.

One of the most important advances in biology has been the discovery that siRNA (small interfering RNA) is able to regulate the expression of genes, by a phenomenon known as RNAi (RNA interference).

2019年9月4日 · Function of siRNA: The main function of siRNA is to protect the cell from exogenous mRNA attacks. Functionally, the siRNA degrades the growing mRNA (exogenous as well as endogenous) and stops gene expression. The origin of the siRNA is exogeneous, it came from viral infections.

An ideal siRNA delivery system should possess the following functions: 1) maintaining siRNA stability in circulation, 2) improving siRNA’s pharmacokinetic characteristics, extending its half-life in the body, and enhancing its distribution in target tissues, 3) promoting cellular uptake of siRNA, 4) facilitating siRNA escape from endosomes, 5 ...

2021年5月13日 · siRNA is a double-stranded RNA molecule that is non-coding. It is also known as silencing RNA and short interfering RNA. It is similar to microRNA (miRNA) and the structure is short and...

2021年8月15日 · The mechanism of action of small interfering RNA (siRNA) is based on post-transcriptional gene silencing. siRNA molecules are usually specific and efficient in the knockdown of disease-related genes. However, they are characterized by low cellular uptake and are susceptible to nuclease-mediated degradation.

Research has shown that siRNA and shRNA are instrumental in the RNA interference (RNAi) pathway, leading to the silencing of specific genes. siRNA is typically introduced directly into cells, whereas shRNA is expressed from a DNA vector, making it …

Two primary categories of these small RNAs— short interfering RNAs (siRNAs) and microRNAs (miRNAs)—act in both somatic and germline line-ages in a broad range of eukaryotic species to regulate endogenous genes and to defend the genome from invasive nucleic acids.

2019年11月6日 · In doing so, siRNAs prevent the production of specific proteins based on the nucleotide sequences of their corresponding mRNA. The process is called RNA interference (RNAi), and may also be referred to as siRNA silencing or siRNA knockdown.

2024年4月10日 · How does siRNA work? SiRNA plays a crucial role in gene silencing by following a multi-step process called RNA interference (RNAi). siRNA is created by cutting long double-stranded RNA molecules into shorter pieces that are then integrated into an RNA-induced silencing complex (RISC).