If only one gene in the sample is being interrogated, a gene-specific primer can yield good results. Otherwise, the best qPCR results came from combinations of oligo(T)s (targeting the poly(A ...

The quality of qPCR and RT-qPCR results can be negatively affected by many experimental variables. To ensure the validity of assay results, sample extraction and preparation steps must be closely ...

However, the accuracy and reproducibility of qPCR results are heavily influenced by the proper use of reference genes as controls. These controls are essential for ensuring that the results reflect ...

Azenta Life Sciences reports how the two-component design of its FrameStar® microplate range reduces thermal expansion and sample evaporation leading to improved consistency of PCR results. Many ...

4. When reporting qPCR results, review the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, as described by Bustin, et al. 3 These guidelines ...

Download this article from Bio-Rad to learn tips and tricks for better assay control management and superior qPCR results. Is the form not loading? If you use an ad blocker or browser privacy features ...

Analyzing results from qPCR centers on an amplification plot, which charts the cycle number against the fluorescent signal. Initial qPCR cycles with minimal amplification and relatively low ...

A direct amplification method, that simplifies and accelerates time to qPCR results, is a good solution to bypass the particularly rate-limiting step of RNA extraction. As the name implies, direct PCR ...