As mentioned previously, melt curve analysis can be used to check the identity of qPCR products, particularly helpful for dye-based amplification. Positive control wells (in a well-designed assay) ...

On the semi-log plot of (log(fluorescence signal) vs. cycle number), the threshold should cross the portion of each amplification curve that appears straight. Determining Which qPCR Reagent ...

qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...

RT-PCR and qPCR: Learning the differences between RT-PCR (used for ... template preparation, and reaction components. Amplification Curves: Interpreting amplification curves to monitor the progress of ...

While the RT-qPCR assay was a gold-standard means of finding respiratory ... are chopped off by probes connected to frameworks. A real-time amplification curve based on the signal change is then ...

A direct amplification method, that simplifies and accelerates time to qPCR results, is a good solution to bypass the particularly rate-limiting step of RNA extraction. As the name implies, direct PCR ...

5. Like all forms of PCR, qPCR and RT-qPCR requires optimization to ensure sufficient and specific amplification. You may need to optimize PCR parameters such as magnesium concentration ...

In this article, we will consider how qPCR works, experimental requirements, data analysis and applications of the technique.