Qdot Western Blotting Kits are designed to be a 'plug-and-play' substitute for colorimetric or chemiluminescent detection systems on blots labeled with rabbit and mouse primary antibodies.

A western blot is a laboratory method used ... The membrane is then incubated with an antibody called the primary antibody, which specifically binds to the protein of interest.

The main steps in a western blot protocol are as follows. Sample preparation, including protein extraction and quantification Protein separation via gel electrophoresis Membrane transfer of proteins ...

high-throughput method for validating the target-specificity of antibodies for the application of western blot. View the full article Also, find out more about how the ENCODE project – an NIH-funded ...