qPCR检测组织虫荷，并系统评估了疫苗对RH、ME49、WH6三种虫株及PRU卵囊的攻击保护效果。 疫苗设计与体外表达 通过生物信息学预测，4x蛋白的亲水区(251-286aa等)和抗原指数区(419-441aa等)显示良好表位特征。Western blot在130kDa处检测到特异性条带，证实4x-mRNA-LNP能在真 ...

通过qPCR（定量PCR）、Western Blot（蛋白印迹）等方法对关键基因的表达结果进行验证，确保数据的可靠性。 二、mRNA表达谱分析的优势1、全面性：mRNA表达谱分析能够覆盖样本中所有mRNA分子的表达信息，揭示基因表达的全景图。 2、高灵敏度：能够检测到低丰度的 ...

Abscisic acid (ABA) prevents cotyledon opening in the dark by modulating splicing through serine/arginine-rich (SR) proteins RS40 and RS41. Light reduces ABA levels, releasing this repression to allow ...

Gene editing could be used to treat millions of glaucoma patients. Glaucoma is a leading cause of blindness, characterized by ...

Myocarditis, or inflammation of the heart muscle, is of particular concern among mRNA vaccine opponents. An analysis published in 2022 found that myocarditis occurred in about 31.2 cases per 1 ...

Methods for tracking RNA inside living cells without perturbing their natural interactions and functions are critical within biology and, in particular, to facilitate studies of therapeutic RNA ...

The qPCR assays can be operated in parallel within a 5184-well nanoliter qPCR (nL-qPCR) chip at a single annealing temperature and buffer condition. We developed a robust bioinformatics approach to ...

A new type of mRNA vaccine is more scalable and adaptable to continuously evolving viruses such as SARS-CoV-2 and H5N1, according to a study by researchers at University of Pittsburgh School of ...

Trans amplifying mRNA vaccine expressing consensus spike elicits broad neutralization of SARS CoV 2 variants. npj Vaccines , 2025; 10 (1) DOI: 10.1038/s41541-025-01166-1 Cite This Page : ...