Until now, studies utilizing mRNA electroporation as a tool for the delivery of tumor antigens to human monocyte-derived dendritic cells (DC) have focused on DC electroporated in an immature state.

New enzyme improves capping efficiency and scalability for mRNA manufacturing workflows, including vaccine generationIPSWICH, Mass., Aug. 9, 2022 ...

mRNA Amplification, sans PCR. By putting two enzymes on the same shift, ... First, reverse transcriptase makes DNA copies of the mRNA, using a chimeric DNA-RNA oligo-dT primer. Next, DNA polymerase ...

In order to reach maturation and be sent to the cellular "machine" that deals with protein synthesis, the non-coding fragments contained in the mRNA, the introns, need to be removed, whereas the ...

This mature form of RNA is called messenger RNA (mRNA). A "post-it" to block protein synthesis In addition to these processes, RNA -- but also DNA molecules -- can undergo a chemical modification ...

For decades, Katalin Karikó's work into mRNA therapeutics was overlooked by her colleagues. ... By 1989, other researchers had found a way to utilise PCR to generate mRNA from scratch, ...

Researchers have focused on a specific type of chemical modification – called methylation – of mRNA molecules in the small worm <i>Caenorhabditis elegans</i>. They found that methylation on a ...

Pillon MC, Sobhany M, Borgnia MJ, Williams JG, Stanley RE. "Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage." Proceedings of the National Academy of Sciences of the United ...

This mature form of RNA is called messenger RNA (mRNA). A "post-it" to block protein synthesis In addition to these processes, RNA - but also DNA molecules - can undergo a chemical modification ...

New England Biolabs (NEB ®) announces the release of the Faustovirus Capping Enzyme (FCE), an mRNA capping solution that combines high activity and a broad temperature range to support mRNA ...