To mitigate the risks associated with handling live TGEV, we utilized the vesicular stomatitis virus (VSV) pseudovirus system to construct a TGEV pseudovirus. The TGEV S gene was recombined into the ...

Using pseudovirus neutralization assays, we evaluated cross-neutralization among major ... At 24 h post transfection, cells were infected with GFP-expressing VSV-G pseudotyped virus (VSV-ΔG) at a MOI ...

7 RBD grew in 0.2 M Sodium chloride, 0.1 M Tris 8.0, 20% w/v PEG 6000. To obtain SARS-CoV-2 PT and variants pseudoviruses, we constructed replication-deficient vesicular stomatitis virus vector ...

The lentivirus VSV glycoprotein gene was replaced with the plasmid expressing SARS-CoV-2 S protein; the lentiviral vector backbone carries the reporter genes for firefly luciferase and green ...

The World Health Organization has listed NiV as a priority pathogen for which vaccines urgently need to be developed. Based on the existing human safety data available for the VSV-EBOV backbone, the ...

Dr. Bloom studies virus evolution at the Fred Hutchinson Cancer Center in Seattle. Viruses far more devastating than the coronavirus that causes Covid-19 have plagued humankind. Smallpox, for ...

The viral particles were collected in the column flowthrough. Neutralization Assay Based on VSV Pseudovirus. Neutralizing activities of antibodies against WT SARS-CoV, WT SARS-CoV-2, WT SARS-CoV-2 ...

Some examples of the viruses that have been used to provide the inner core genome for the generation of SARS-CoV-2 pseudoviruses include the vesicular virus of vesicular stomatitis virus (VSV), as ...