The TaqMan and SYBR Green I-based one-step real-time quantitative RT-PCR assays have potential utility for detection and quantification of CHIKV. These assay systems have been demonstrated to be ...

为解决利什曼病临床诊断中显微镜检查准确性有限和不便的问题，研究人员开展了利用 TaqMan 定量 PCR（qPCR）检测临床样本中皮肤和内脏利什曼病的研究。结果显示该方法灵敏度 96.3%、特异性 100.00% 、准确率 98.85%，对利什曼病诊断意义重大。 利什曼病是一种被 ...

Total RNAs were extracted using the RNeasy micro kit (QIAGEN) according to the manufacturer’s protocols. Sample quantity and purity, reverse transcription, pre-amplification, and high-throughput qPCR ...

为检测 Bopivirus B 并调查其感染情况，研究人员建立 TaqMan qPCR 方法，发现其流行广泛且与腹泻有关。 在动物健康领域，病毒的肆虐常常让养殖从业者们头疼不已。近年来，一种名为 B 型博尔纳病毒（Bopivirus B）的新型病毒逐渐进入人们的视野，它属于微小核糖 ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial ...

and Taqman Gene Expression Mastermix (Applied Biosystems) were mixed and aliquoted into a qPCR plate. Following primers and probe used were used: HSV Pol F: 5’-ACCGCCGAACTGAGCAGAC-3’, HSV Pol R: ...

TaqMan® probes have traditionally been used for this technique, but their expense and the required complexity in assay design are significant drawbacks. EvaGreen® is less likely to inhibit PCR and ...

She is passionate about making complex scientific concepts accessible to readers. View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular ...

PCR reactions were performed on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) in fast mode with the following protocol: initial denaturation ... final concentrations of 1 × GoTaq ...