引入丙炔基：利用丙炔基卤代物（如 propargyl bromide）在碱性条件下与PEG1–S–H进行亲核取代，获得 Propargyl-PEG1-SS-PEG1-OH。 酯化保护：将末端羟基通过与叔丁基溴乙酸反应或酸性条件下与异丁烯酸反应，形成 t-butyl 酯保护基，得到终产物 Propargyl-PEG1-SS-PEG1-t-butyl ester。

Acid-PEG25-t-butyl ester Acid-PEG12-t-butyl ester Acid-PEG10-t-butyl ester Acid-PEG3-PFP ester 平台声明：该文观点仅代表作者本人，搜狐号系信息发布平台，搜狐仅提供信息存储空间服务。

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LC-MS/MS grade, methanol, acetonitrile, methyl tert-butyl ether, and formic acid (99.5%) were products of J. T. Baker (Phillipsburg, NJ, USA). Water was obtained using a Milli-Q system (Millipore, ...