In previous work, we sought to identify proteins that associate with SIMR-1 and ultimately found that SIMR-1 associates with HRDE-2 and the nuclear Argonaute protein, HRDE-1, to promote correct HRDE-1 ...

Argonaute蛋白的PIWI结构域具有RNaseH样的折叠情况，这说明Argonaute蛋白应该是具有切割活性的。和RNaseH酶一样，Argonaute蛋白同样也需要二价金属离子的辅助来完成切割反应，从而生成3’端是自由羟基基团的RNA产物的5’端，和5’端是磷酸基团的RNA产物的3’端。

Most metazoan genes are regulated post-transcriptionally by RNA interference (RNAi) (Friedman et al., 2009).RNAi is a conserved process whereby an Argonaute (Ago) protein binds to a small RNA, such as ...

R2TP/Prefoldin-like. R2TP was initially identified in yeast as an Hsp90- associated multiprotein complex (R2TP-Hsp90 complex) (Zhao et al., 2005).This complex is well conserved from yeast to humans ...

The crystal structure of MpAgo bound to a guide RNA reveals the distinct coordination of the terminal 5′-hydroxyl group and how the canonical Argonaute fold has ... DE Weinberg, DP Bartel, DJ Patel, ...

For small RNA duplex loading, Argonaute proteins are believed to first undergo a conformational change to open the small RNA duplex binding pocket (Kawamata et al., 2009; Yoda et al., 2010).This ...

The binding affinity of recombinant hAgo2 or binary hAgo2/guide RNA complexes in binding buffer (10 mM Tris, pH ... DP Bartel, DJ Patel, Structure of yeast Argonaute with guide RNA. Nature 486, ...

Researchers at the Whitehead Institute and Memorial Sloan-Kettering Cancer Center have defined and analyzed the crystal structure of a yeast Argonaute protein bound to RNA. This complex plays a ...

CAMBRIDGE, Mass. (June 20, 2012) – Researchers at the Whitehead Institute and Memorial Sloan-Kettering Cancer Center have defined and analyzed the crystal structure of a yeast Argonaute protein ...