First we must transfer the data from table 3 and 4 into the purification table. Make sure to use the values of protein and enzyme concentration for each original, undiluted fraction; also note the ...

pH is a measure of how acidic or alkaline a solution is. The diagram below shows what happens to an enzyme when denaturation occurs. Each enzyme has an optimum pH but it also has a working range ...

We had at our disposal not only an enzyme assay but also a highly ... is important in the overall inhibitory activity by providing strong and specific interactions with the different 'pockets ...

(Image: National Human Genome Research Institute) Enzymes are highly specific to their substrates, the molecules upon which they act ... and providing an optimal environment for the reaction. Enzyme ...

After this synthesis had been completed, preparation of Nα-benzoyl-dl-arginme-β-naphthylamide hydrochloride via the carbodiimide procedure was reported (cf. ref. 2). Gomori, G., J. Histochem ...

This is shown in the graph below. In the graph above, as the pH increases so does the rate of enzyme activity. An optimum activity is reached at the enzyme’s optimum pH, pH 8 in this example.