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Cell culture expansion for biotherapeutic development has traditionally involved ... the entire cell expansion process takes about three to four weeks and starts with a vial of viable cells ...
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For reproducibility, the pre-culture was passaged in the exponential phase two or three times before starting any of the following cultivation experiments. Shake flask cultivation was performed as ...
was used to increase the information content from shake flask experiments. The OTR of a Chinese hamster ovary cell line was monitored by applying electrochemical sensors. Glass flasks stoppered with ...
Review: Why shake or stir cells and an outline of system specific differences Learn how to identify the right cultivation method, vessel type, vessel material, and instrument configuration for your ...