Background: Non-small-cell lung cancer (NSCLC) is the most common histological subtype of lung cancer and the leading cause of cancer-related deaths worldwide. Targeted therapies and immunotherapies ...

3D cellular-specific epigenetic and transcriptomic reprogramming is critical to organogenesis and tumorigenesis. Here, we dissect the distinct cell fitness in 2D (normoxia vs. chronic hypoxia) vs 3D ...

Tumor Protein 53 (p53) expressed from gene TP53 located on the short arm of chromosome 17 is one of the seminal tumor suppressors. 1 - 3 p53 dysfunction occurs through a multihit process, typically ...

Place the RNeasy MinElute spin column in a new 1.5-mL collection tube. Add 14 µL of RNase-free water directly to the center of the spin column membrane. Close the lid gently and centrifuge for 1 min ...

Cells were incubated with or without Kp396 at a MOI of 0.4 in the killing assay media (IMDM media supplemented with 10% FBS, 292 μg/ml L-glutamine, 1x MEM non-essential amino acids, 10 mM HEPES, and 1 ...

The adaptive T cell response is accompanied by continuous rewiring of the T cell’s electric and metabolic state. Ion channels and nutrient transporters integrate bioelectric and biochemical signals ...

RNA was extracted with QIAzol and RNeasy Mini kit (Qiagen) according to the manufacturer’s recommendations, including a DNase treatment. Sequencing libraries were prepared with NEXTflex Rapid ...

Preparation of total RNA from sessile cells using the RNeasy Mini Kit When enzymatic (Figure 2A) or mechanical lysis (Figure 2B) was used to obtain total RNA from sessile cells, the results were ...

Plasma membrane rupture (PMR) is a defining and terminal event in many forms of cell death (Zhang et al, 2018; Hiller and Broz, 2021). While it was long thought to occur passively as a result of ...

注意事项： 第一次使用RNeasy Kit，请阅读说明书的“Important Notes” (page 18)。 第一次操作RNA，请阅读说明书Appendix A (page 63)。 为了得到最好的结果，收集到的组织应该立即置于RNA稳定剂RNAlater中 (见page 36)。 稳定于其中的组织可以在37°C 保存1天， ...

The RNeasy PowerMax Soil Pro Kit, designed for isolating high-purity RNA from challenging soil samples rich in PCR inhibitors using QIAGEN’s advanced Inhibitor Removal Technology.