Cell apoptosis was detected by flow cytometry with Alexa Fluor 488 annexin V and propidium iodide fluorescence staining (Molecular Probes, Eugene, Oregon).

Annexin V Alexa Fluor 488 染色液：用于标记细胞膜上的磷脂酰丝氨酸（PS），识别早期凋亡细胞。 碘化丙啶（Propidium Iodide, PI）：用于区分晚期凋亡细胞和坏死细胞。 结合缓冲液（Binding Buffer）：用于维持细胞膜的完整性，确保Annexin V与PS的结合。 用途：可以用于细胞 ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Metastasis is the primary cause of death in ...

Depending on the assay, the cells were exposed to DADS or EC alone or in combination ... Flow cytometry analysis was performed by using the Dead Cell Apoptosis Kit with Annexin V-FITC and Propidium ...

In the CCK-8 assay, cells are generally planted in a 96-well plate and exposed to various ... The samples were treated with fluorescently-labeled Annexin V and Propidium Iodide (PI) in the absence of ...

For example, the BCA assay is one that combines the reduction of Cu2+ into Cu1+ by proteins in an ... Fluorescent dyes may also be used to detect apoptosis and for cytotoxicity studies. Annexin V ...

Finally, the mitochondrial membrane potential was analyzed by flow cytometry, and the results were obtained using ImageJ software. Annexin V-fluorescein isothiocyanate/propidium iodide staining assay ...

Ltd. (Fuzhou, China). Cell counting kit-8 (CCK-8), apoptosis detection kit (including Annexin-V fluorescein isothiocyanate, Annexin-V FITC, and propidium iodide, PI), and JC-1 mitochondrial membrane ...