Site-directed mutagenesis was employed to replace the Oct-1 phosphorylation sites (Ser/Thr) by alanine using PfuTurbo (Stratagene, La Jolla, CA, USA). All mutagenized cDNA were sequenced entirely.

May 1, 2008 (Vol. 28, No. 9) Ti-Tyr Chips Deliver Quantitative Analyses Site-specific tyrosyl phosphorylation is a key mediator of intracellular communication and represents a rapid readout of the ...

Purpose Akt is a serine/threonine kinase that has been implicated in lung tumorigenesis and lung cancer therapeutic resistance. Full activation of Akt requires two phosphorylation events, but only one ...

Two different models were used to answer this question of tau phosphorylation master sites. The team discovered the role of these master sites in health and disease in the gene therapeutic approach ...

Although serine-473 phosphorylation and dual serine-477/threonine-479 phosphorylation both lead to active forms of the enzyme, the two forms act upon their substrates with different catalytic ...

Phosphorylation site identification, our newly added Mass Spectrometry service, includes the enrichment of phosphorylated peptides, and phospho-site i ...

Phosphorylation of either S473 or T308 was positive in most NSCSLC specimens, but was detected rarely in surrounding normal tissues. When Akt activation was defined by using both sites of ...

The researchers demonstrated the power of the method by studying protein histidine phosphorylation in Escherichia coli.They detected 2,129 phosphorylation sites, which is about 10 times as many as ...

Notably, this relationship between different phosphorylation sites, which the authors dubbed interdependency, was most pronounced in the presence of the MAP kinase p38α, with other kinases more weakly ...

Study: Emergence of glycogen synthase kinase-3 interaction domain enhances phosphorylation of SARS-CoV-2 nucleocapsid protein.Image Credit: Juan Gaertner/Shutterstock. There are four key ...