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蛋白质分析:通过蛋白质免疫印迹(Western blotting)、免疫共沉淀(Co - immunoprecipitation)实验,分析蛋白质的表达、稳定性及相互作用。 染色质分析 :采用染色质免疫沉淀测序(ChIP - seq)、染色质免疫沉淀 PCR(ChIP - PCR)技术,确定 VRN2 结合位点和 H3K27me3 修饰水平。
Western blot analysis confirmed that expression of 2×Flag–Eed in these cells was moderate, and did not substantially elevate overall Eed levels (Supplementary Information, Fig. S1a).
What is western blotting? In 1975, Southern invented a new method that enabled analysis of DNA identity, size and abundance. It involved the use of electrophoresis to separate DNA fragments based on ...
If it’s mutated, cells can grow uncontrollably, and cancer can arise—a fact that has made PRC2 a source of keen interest for drug developers. New research by scientists at CU Boulder and Harvard ...
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