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蛋白质分析:通过蛋白质免疫印迹(Western blotting)、免疫共沉淀(Co - immunoprecipitation)实验,分析蛋白质的表达、稳定性及相互作用。 染色质分析 :采用染色质免疫沉淀测序(ChIP - seq)、染色质免疫沉淀 PCR(ChIP - PCR)技术,确定 VRN2 结合位点和 H3K27me3 修饰水平。
在拟南芥(Arabidopsis thaliana)中,PRC2 存在多种类型,其中 VRN2 - PRC2 参与调控春化作用 ... 蛋白质分析:通过蛋白质免疫印迹(Western blotting)、免疫共沉淀(Co - immunoprecipitation)实验,分析蛋白质的表达、稳定性及相互作用。 染色质分析:采用染色质免疫沉淀 ...
Figure 1: Jarid2 selectively associates with PRC2 proteins in mouse ES cells ... using calcium phosphate. Western blot analysis. Western blots were carried out using the following antibodies ...
The western blot may appear to be just another staple lab technique. However, there are some stories surrounding its invention that are definitely worth knowing. This might be news to some: scientists ...
If it’s mutated, cells can grow uncontrollably, and cancer can arise—a fact that has made PRC2 a source of keen interest for drug developers. New research by scientists at CU Boulder and Harvard ...
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