In addition to its long-studied presence in the cytoplasm, actin is also found in the nuclei of eukaryotic cells. The function and form (monomer, filament, or noncanonical oligomer) of nuclear actin ...

Here, we report the biochemical characterization of human cytoskeletal γ-actin carrying mutation E334Q, a mutation that leads to a hitherto unspecified non-muscle actinopathy. Following expression, ...

Together our studies reveal that PGs play an important role in limiting the level of nucleolar actin and regulating its form–G-actin vs. C4 G-actin and monomeric vs. polymeric actin (AC15). We ...

These studies unraveled a cooperation with ADF/Cofilin and Twinfilin in F-actin disassembly, a nucleotide exchange activity on globular actin monomers (G-actin) that is required for F-actin assembly ...

Asgard profilins were demonstrated to regulate actin filament nucleation. Here, we identify actin filament severing, capping, annealing and bundling, and monomer sequestration activities by gelsolin ...

Sequestration does not affect the monomer steady-state value Profilin is a major regulator of actin assembly that binds ATP–G-actin with high affinity. Profilin–actin exclusively feeds barbed ...

Figure 2: Classic model of F-actin network regulation. As the filament elongation rate is directly proportional to the concentration of polymerizable monomers, the rate that F-actin is recycled ...

Thymosin-β4 (Tβ4) sequesters actin monomers to help maintain the high concentrations of unpolymerized actin in higher eukaryotic cells. Despite more than two decades of research investigating the ...

Standing in front of dozens of uniformed first responders, President Obama on Tuesday warned Congress that “hundreds of thousands of people will lose their jobs if a March 1 sequester is allowed ...

It is unclear whether cofilin contributes to stimulus-induced actin filament assembly by supplying actin monomers for polymerization or by creating free barbed ends through its severing activity. By ...

The WH2 (Wiscott–Aldridge syndrome protein homology domain 2) repeat is an actin interacting motif found in monomer sequestering and filament assembly proteins. We have stabilized the prototypical WH2 ...