One involves studying the effect of a 'candidate' gene on a phenotype; for example, using transgenesis or homologous recombination (creating 'knockout' or 'knockin' mice). The other entails using ...

We describe here a detailed protocol for generating gene knockout rats by homologous recombination in embryonic stem (ES) cells. This protocol comprises the following procedures: derivation and ...

Homologous recombination plays essential roles in the repair of DNA double strand breaks (DSBs) and collapsed replication forks. The fundamental reaction in homologous recombination is the exchange of ...

It is thought that these proteins play a role in preventing recombination during DNA replication when it is not needed. An alternate hypothesis proposes that homologous chromosomes reside in pairs ...

The Transgenic and Knockout Core can develop mice with targeted mutations ... produced by first modifying a given endogenous gene locus in embryonic stem cells via homologous recombination. Embryonic ...