20 µg/ml (200 ng) of DNA was incubated with increasing concentrations (10–200 ng) of proteins (thrombin, prothrombin) or peptides (GKY25, IVE25, or LL-37) in 10 mM Tris, pH 7.4 for 1 hour at 37 °C.
Also, analyze the products of the small-scale reaction by alkaline agarose gel electrophoresis and store ... and 1 μl E. coli DNA ligase (1,000–4,000 units/ml). Incubate the reaction at 15 ...
DNA was extracted from all spiked and non-spiked stool samples in triplicates ... From each reaction, 5 μl were analyzed by 2% agarose gel electrophoresis. PCR products were purified using AMPure XP ...
Then, in 1986, a publication by the Organization for Economic Cooperation and Development (OECD), called "Recombinant DNA Safety Considerations," became the first intergovernmental document to ...
A teacher at Drakensburg Secondary School in South Africa is under investigation for allegedly cutting off a Hindu student's religious thread. The South African Hindu Maha Sabha has condemned the ...