A multimodal imaging study combines cryo-X-ray nanotomography and super-resolution fluorescence microscopy to reveal how ...

Abstract: The entry process of virus particles into cells is decisive for infection. In this work, we investigate fusion of virus particles with the cell membrane via time-lapse fluorescence ...

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that provides reliable imaging biomarkers for the diagnosis of cancer and genetic diseases at the cellular level. An ...

Super-resolution fluorescence ... cell that cryo-ET electrons can penetrate. Then the scanning electron microscope shoots electrons at the sample and produces those nice, high-resolution cryo-ET ...

Cryogenic electron tomography (cryo-ET) combined with subtomogram averaging, allows in situ visualization and structure determination of macromolecular complexes at subnanometre resolution. Cryogenic ...

Another, the flat-to-curved transition model, says that a flat lattice of clathrin molecules first assembles on the inside of the cell membrane, followed by a conformational change to form the ...

Researchers used a sophisticated fluorescence microscopy ... for both models can be seen by electron microscopy -- but those are static snapshots of fixed cells that do not reveal the actual ...

Volume or three-dimensional (3D) correlative light and electron microscopy ... a CLSM image is collected of the same lysosomes and the ER and, additionally, the fiducial particles emitting in another ...

but it can’t be used on living cells because the intensity of the electron beam destroys the sample. Other methods, like fluorescence microscopy, don’t harm the sample but come at a cost to ...

Genetic tags allow rapid localization of tagged proteins in cells and tissues. APEX ... This method produces an easily detectable gold particle at the site of the fusion protein with high resolution ...

compressed FLIM has a striking advantage in acquiring a widefield lifetime image within a single camera snapshot, thereby eliminating motion artifacts and enabling fast recording of biological events.

we applied correlative fluorescence microscopy (FM), and scanning transmission electron microscopy (STEM) to image specifically labeled SKBR3 breast cancer cells. The breast cancer cells were grown on ...