28-30 Chemical reagents were purchased from different commercial sources and used without further purification. Acetone, hexane, dichloromethane, chloroform, ethyl acetate, methanol and isopropanol ...

Other chemicals were obtained commercially and were of reagent grade. An aliquot of 100 μl of 1.25 mg ml −1 different glycosaminoglycan substrates [HA, chondroitin sulfate type A (CSA), chondroitin ...