Here we present the structure of a full-length human TRPM4 bound with the agonist Ca 2+ and the modulator DVT at an overall resolution of 3.8 Å using single-particle electron cryo-microscopy ...

where he had used a cryo-electron microscope and more conventional averaging techniques to discern the 3-D structure of LDL -- the "bad cholesterol" thought to be a major risk factor for heart ...

What is Cryo-Electron Microscopy? Cryo-electron microscopy (cryo-EM) is a powerful technique used to determine the three-dimensional structure of biological macromolecules, such as proteins and ...

To create them, the team sequentially painted different cellular structures with precipitates of rare earth metals like lanthanum, cerium and praseodymium, which allows the electron microscope to ...

Using cryo-electron ... a crown-like structure with sixfold symmetry on the outside of the DMV. By attaching protein labels that add extra mass, which can be seen in the electron microscopy ...

The Electron Microscopy shared resource offers techniques that allow researchers to resolve structures from cellular ultrastructure all the way down to molecular interactions. Our expertise in EM ...

Cryo-electron microscopy means that the proteins are cooled down to -170°C in an ultra-thin layer, after which they are "X-rayed" with a powerful beam of electrons and form small shadows on an ...

As in cryoEM, samples are frozen in a hydrated state, preserving their structure, then subjected to a beam of electrons in a transmission electron microscope. Unlike cyroEM, micro-electron diffraction ...