DAPI can be used to stain nuclei using fluorescent microscopy ... First the sample is fixed using an appropriate fixation protocol. After fixing, the sample is equilibrated with PBS.

The protocol can be carried out with multiple ... For nuclear staining, add 100 μl of DAPI staining buffer to the tissue section. Incubate the slides at room temperature for 15 min.

Protocol - PI staining Use of RNase with PI protocol Protocol - DAPI staining Protocol - 7-AAD staining UV excitable DNA dyes such as DAPI and Hoechst 33342 are normally excited with a UV laser ...

Here, we developed a protocol for the simultaneous detection of plasma membrane integrity (based on DAPI) or nuclear morphology (based on Hoechst 33342), mitochondrial functions (based on the ...

Current offerings of the most commonly used dyes, DAPI and Hoechst 33342, usually require time-consuming weighing and/or multiple tedious dilution steps. Bio-Rad’s PureBlu Nuclear Staining Dyes are ...