Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these ...

aDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA, 94305, USA bDepartment of Radiation Oncology, Wake Forest University School of Medicine, Winston Salem, NC, ...

As an example, DAPI staining identifies cell nuclei, while CD20 staining helps segment cell membranes in MxIF. However, a persistent challenge in MxIF is saturation artifacts, which hinder single-cell ...

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pelleted twice and subsequently resuspended in HBSS with 2% FBS + DAPI (1 μg/ml; D9542, Sigma‐Aldrich). For intracellular staining, cells were first stained live for cell surface markers and then ...

4′,6-diamidino-2-phenylindole (DAPI). We also test the influence of protocols on different antibodies raised against the same protein target and finally, explore whether IHC protocols optimized in ...

To date, assessing the staining quality in electron microscopy requires running a sample through the entire staining protocol end-to-end, which can take weeks or even months for large samples, ...

Whole-mount immunofluorescence staining ... with DAPI (blue) and the actin cytoskeleton with phalloidin (white). See Co et al. Organoid PTM signaling network from the TOBis MC protocol A post ...

Troubleshooting H&E stains can be difficult and frustrating. Our objective is to highlight some of the most common mistakes when choosing staining reagents and creating protocols. By minimizing them, ...