Staining patterns of MMR proteins were evaluated using normal epithelial, stromal and inflammatory cells as internal controls. Stained slides were scored as: (1) positive—that is, showing nuclear ...

Zebrafish larvae were used to detect intracellular ROS formation using DCFDA staining. The DCFDA staining procedure was performed according to a previously published protocol (Singh et al., 2022). The ...

For the detection of intracellular cytokine production, PBMCs were stimulated with 100 ng/mL IL-33 for 24 hours prior to flow cytometry analysis. The intracellular cytokine staining procedure was ...